Rab27在的细胞外囊泡生物发生和感染
L Rainer Butler1, Nisha Singh1, Liron Marnin1
1The University of Maryland Baltimore, Baltimore, Maryland, USA.
bioRxiv : the preprint server for biology
|November 14, 2023
概括
Rab27对黑腿的生存和食至关重要. 在中抑制Rab27减少了Anaplasma fagocytophilum的获取,并改变了细胞外囊泡的大小,影响了传播疾病的传播.
科学领域:
- 载体生物学 载体生物学
- 分子寄生虫学 分子寄生虫学
- 细胞生物学 细胞生物学
背景情况:
- 黑腿 (Ixodes scapularis) 在美国传播许多媒介性疾病,包括Anaplasma phagocytophilum.
- 的细胞外囊泡 (EVs) 可能会影响病原体的传播,但它们在关节动物中的生物发生不明.
- RabGTPases是哺乳动物EV生物发生的关键调节者,但它们在虫中的作用尚不清楚.
研究的目的:
- 研究Rab27,一个RabGTPase在Ixodes scapularis细胞外囊泡 (EVs) 生物发生中的作用.
- 为了确定Rab27对的健康,食和细胞囊的获取的影响.
主要方法:
- 使用in silico分析 (BLAST) 重建了 EV生物发生途径,并确定了Rab27.
- 在Ixodes scapularis中使用RNA干扰 (RNAi) 在细胞系 (ISE6) 和女中沉默Rab27.
- 从沉默的细胞中收集了EV,并分析了它们的尺寸配置.
- 在小鼠身上用沉默的Rab27养母,以评估的养和Anaplasma 细胞菌的获取.
主要成果:
- 在分析中,Rab27被确定为虫EV生物生成中的关键蛋白质.
- 在Ixodes scapularis中沉默Rab27对的适应性产生了负面影响.
- 沉默Rab27的获得的Anaplasma phagocytophilum显著减少.
- 在细胞中击败Rab27改变了分泌的EVs的大小分布.
结论:
- Rab27对于的EV生物发生是必不可少的,影响EV大小.
- 拉布27在成功食和获得阿纳普拉斯马细胞粒的过程中起着至关重要的作用.
- Rab27是,宿主和病原体之间的复杂相互作用的关键调节者.
更多相关视频
09:36Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
Published on: August 26, 2021
3.8K
10:29Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
Published on: July 6, 2016
10.7K
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.5K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.5K
Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
Receptor-mediated Endocytosis
6.1K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.1K
SNAREs and Membrane Fusion
10.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.9K
Overview of Secretory Vesicles
8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K
