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如何在Polycomb蛋白中的一个无序链接器 Polyhomeotic调节相位分离和寡合化

Tim M Gemeinhardt^1,2, Roshan M Regy³, Tien M Phan³

¹Montreal Clinical Research Institute (IRCM), Montreal, QC, Canada.

bioRxiv : the preprint server for biology

|November 14, 2023

概括

蛋白质链接体的进化变化改变了生物分子凝结物的特性. 这些序列变化会影响多体质 (Ph) 复合体对染色质的调节,影响细胞中的凝结物形成和动态.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

生物分子凝结物组织细胞过程,包括染色质功能.
蛋白质序列决定了凝结物质的特性,但根本的机制尚不清楚.
跨物种的比较揭示了保存的功能和进化差异.

研究的目的:

为了研究多同源 (Ph) 蛋白的进化序列变化如何影响生物分子凝结物形成和特性.
为了比较无菌阿尔法基因 (SAM) 域及其在 *Drosophila* 和人类PRC1复合体中的无序链接器.
了解蛋白质序列,寡合化和凝结物特征之间的联系.

主要方法:

*在体外* 溶解试验.
分子动力学模拟.分子动力学模拟.
在 *Drosophila* 和人类细胞中进行细胞测试.
对 *Drosophila* 形象性盘发育的分析.

主要成果:

在保存的SAM域和Ph的无序链接器之间存在着进化上不同的联系.
链接器-SAM相互作用调节蛋白质的寡合化,影响凝结物的形成和特性.
寡合化影响了凝结物动态,但对形成的影响很小.
链体-SAM相互作用影响凝结物形成 *in vitro* 和 *in vivo*,并影响 *Drosophila* 的发展.

结论:

连接保存域的无序链接器中的进化序列修改可以改变生物分子凝聚物质的特性.
这些发现提供了关于序列分离如何塑造细胞组织和调节的见解.
这项研究强调了链接区域在调节PRC1.1.等染色体调节者的功能方面的重要性.

关键词:

寡合化是指一种氧化物.多重复合的多重复合.无菌的阿尔法基因 (SAM)分析超离心 (AUC) 的方法生物化学生物化学生物分子凝聚剂是生物分子的凝聚物.基因调节基因调节基因调节本质上是无序的区域 (IDR).分子动力学模拟,分子动力学模拟阶段分离的阶段分离.

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