低温电磁和AFM可视化了线性多基化物:单个链和单链组合与蛋白质

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Alexander Andrianov1, Raman Hlushko1, Edvin Pozharski2

  • 1University of Maryland.

Research square
|November 14, 2023
PubMed
概括

可生物降解的聚合基酸与疫苗的蛋白质抗原自组装. Cryo-EM揭示了它们的结构以及蛋白质结合如何影响复杂的形成,为生物活动提供了洞察力.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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