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一个Osmr条件淘汰赛鼠标模型的表征
Logan S Schwartz1,2, Ruth L Saxl1, Tim Stearns1
1The Jackson Laboratory, Bar Harbor, ME, USA.
bioRxiv : the preprint server for biology
|November 14, 2023
概括
这项研究研究了使用条件淘汰赛小鼠模型在成年人血液形成中的奥诺斯塔丁M受体 (OSMR) 信号传递. 结果显示,OSMR对整个组织的mRNA和蛋白质表达具有可变的影响,因此需要谨慎使用.
科学领域:
- 免疫学 免疫学 免疫学
- 血液形成 血液形成 血液形成
- 分子生物学分子生物学
背景情况:
- 昂哥斯塔丁M (OSM) 是一种具有双重作用的IL-6家族细胞因子.
- OSM信号传输涉及包括OSMRβ.在内的受体综合体.
- 了解成年人血液形成中的OSM-OSMRβ至关重要.
研究的目的:
- 描述条件的Osmr小鼠模型用于研究成年人造血.
- 研究OSMR2外因子删除对血造和非血造组织中OSM信号传递的影响.
主要方法:
- 利用Osmr小鼠模型与Mx1-Cre交叉用于成人造血细胞的诱导性重组.
- 在血液造血干细胞中分析了基因重组,mRNA表达 (TaqMan试验),蛋白质水平 (西方斑) 和RNA-seq.
主要成果:
- 在多种组织中观察到的Osmrfl等位基因的完全重组.
- 在不同组织 (骨髓,肝脏,肺,脏) 中OSMR转录和OSMR蛋白水平的可变降低.
- 从淘汰赛小鼠的造血干细胞中部分抑制OSM刺激的基因.
结论:
- B6;129-Osmr/J条件小鼠模型表现出对Osmr mRNA和蛋白质表达的组织依赖性影响.
- 在使用此模型时建议谨慎使用,因为基因删除效率和下游信号影响是可变的.
- 需要进一步的表征才能充分理解成年人血液形成中的OSM-OSMRβ信号.
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