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在宏道至数字微流体平台上进行综合症诊断试验,用于自动识别多种呼吸道病原体

Cheng Dong¹, Fei Li^2,3, Yun Sun³

¹School of Intelligent Systems Science and Engineering/JNU-Industry School of Artificial Intelligence, Jinan University, Zhuhai 519000, China.

|November 14, 2023

概括

一个新的,负担得起的微流体芯片系统能够快速地在现场检测COVID-19和其他呼吸道病毒. 这种"样本入答案"平台为资源有限的地区提供了低成本的解决方案,改善了诊断的可访问性.

科学领域:

生物技术和生物医学工程生物技术和生物医学工程
传染病诊断传染病诊断传染病诊断
微流体学微流体学

背景情况:

随着COVID-19的爆发,人们越来越需要可访问的诊断工具.
核酸放大测试 (NAT) 对于检测SARS-CoV-2至关重要,但通常需要集中设施.
资源有限的环境缺乏负担得起的,对传染病的现场诊断系统.

研究的目的:

开发一个低成本的,集成的"样本-在-答案-out"微流体系统用于核酸检测.
为了在现场诊断呼吸道病毒,包括SARS-CoV-2,流感A/B,在护理点.
创建一个适合资源有限地区和大规模生产的平台.

主要方法:

一个宏频道到数字的微流体芯片被设计用于自动核酸提取,净化和放大.
该系统使用基于珠子的提取和逆转录量的聚合酶链反应 (RT-qPCR) 在数字微流体液滴中.
该芯片集成了一个宏道接口用于样本输入和多个光通道用于检测.

主要成果:

开发的微流体平台在综合评估中表现出卓越的性能.
临床样本显示100%与SARS-CoV-2和流感A/B检测的离芯片诊断一致.
该系统对于一次性使用具有成本效益,制造业已准备好进行大规模制造.

结论:

宏观通道到数字的微流体平台符合医疗点检测 (POCT) 的要求.
主要优势包括低成本,易于使用,高灵敏度和特异性,以及大规模生产能力.
这项技术为各种环境中可访问的传染病诊断提供了可行的解决方案.

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