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通过调节NOR1,NRF1减轻了质母细胞细胞的氧化应激

Jiali Wang¹, Shuai Chen², Wang Xiang³

¹Department of Colon and Rectal Surgery, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, China.

Folia biologica

|November 14, 2023

概括

核呼吸因子1 (NRF1) 调节质母细胞瘤 (GBM) 中含有氧化-酸盐域的蛋白1 (NOR1). 低表达的NOR1和NRF1与GBM相关,NRF1通过上调NOR1.1,防止氧化应激.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
神经科学是一个神经科学.

背景情况:

氧化酸域含有蛋白1 (NOR1) 是一种重要的瘤抑制剂,但其在质母细胞瘤 (GBM) 氧化应激中的作用尚未完全理解.
研究NOR1在GBM氧化应激中的调节机制对于了解疾病进展至关重要.

研究的目的:

评估GBM和正常脑组织中NOR1和核呼吸因子1 (NRF1) 的表达.
在GBM细胞中在氧化应激条件下研究NOR1和NRF1之间的关系.
阐明NRF1-NOR1轴在GBM细胞增殖,入侵,迁移和亡中的功能作用.

主要方法:

定量实时聚合酶连锁反应 (qRT-PCR) 和西部斑点 (WB) 用于基因和蛋白质表达分析.
细胞计数套件-8,Transwell,以及伤口愈合分析,以评估细胞的增殖,入侵和迁移.
流细胞计,TUNEL染色和生物化学测试来评估亡,活性氧物种 (ROS),超氧化物失调酶和催化酶活性.
染色体免疫沉 (ChIP) 试验证实NRF1与NOR1.1结合.

主要成果:

与正常大脑组织相比,NOR1和NRF1表达水平在GBM组织中显著较低,并且具有积极的相关性.
H2O2诱导的氧化应激减少了NRF1和NOR1的表达,并在U251 GBM细胞中增加了ROS水平.
奇普测试证实NRF1直接与NOR1结合.
过度表达NRF1减轻了氧化应激对U251细胞增殖,迁移和入侵的负面影响,而NOR1敲击逆转了这种保护作用.

结论:

在GBM中,NRF1充当了NOR1表达的积极调节者.
NRF1-NOR1通路对GBM中氧化应激诱导的损伤起着保护作用.
针对NRF1-NOR1轴可能为GBM治疗提供治疗策略.

关键词:

在GBMGBMGBMGBMGBM 没有NOR1 没有NOR1 这就是NRF1的NRF1.氧化应激是一种氧化应激.

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