细菌纤维素电纤维网覆盖着基纳米纤维用于鼓膜修复
Bahareh Azimi1, Atefeh Rasti2, Alessandra Fusco3
1Department of Civil and Industrial Engineering, University of Pisa, Pisa, Italy.
Tissue engineering. Part A
|November 14, 2023
概括
这项研究开发了一个生物基的纳米纤维贴片从细菌纤维素和基纳米纤维的耳膜修复. 环保的脚手架是不刺激的,细胞相容,并促进伤口愈合.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 耳膜 (TM) 穿孔,通常是由于慢性中耳炎,在耳科手术中提出了重大临床挑战.
- 潜在的感染可能会阻碍自然的自我愈合过程,需要先进的治疗策略.
- 开发生物相容和生物活性支架对于促进有效的TM修复至关重要.
研究的目的:
- 开发一种新的生物基和生物活性纳米纤维贴片用于耳膜 (TM) 修复.
- 为了利用使用绿色溶剂加工的细菌纤维素 (BC) 和胺纳米纤维 (CNs) 来制造脚手架.
- 评估开发的脚手架的细胞相容性,生物相容性和伤口愈合潜力.
主要方法:
- 制造电BC纳米纤维网格,通过电喷涂通过CNs进行表面修饰.
- 对CN/BC网格的形态,物理化学和机械性能进行全面的描述.
- 用L929纤维细胞进行体外细胞毒性测定和使用HaCaT和HUVEC细胞进行细胞培养研究.
- 在子皮肤上的体内生物相容性评估,以及对抗微生物和伤口愈合特性的评估.
主要成果:
- 开发的CN/BC纳米纤维网表现出极好的细胞兼容性 (97.8%的细胞活力),并且在体内无刺激作用.
- 角质细胞和内皮细胞都在支架上成功生长,这表明它们适合于TM愈合模型.
- 存在的CN增强了间接的抗菌活性,并提高了HaCaT细胞中关键的促炎细胞因子的调节,这对伤口愈合至关重要.
- 脚手架展示了一种细胞因子表达途径,与伤口愈合相兼容,并刺激了抗微生物的表达.
结论:
- 该研究为TM维修应用提供了一个有希望的,自然的和生态可持续的纳米纤维网.
- BC和CN的协同作用增强了组织再生和愈合潜力.
- 开发的支架是解决TM穿孔的可行候选者,即使存在感染.
相关概念视频
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Fibril-associated Collagen
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For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
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Fibronectins Connect Cells with ECM
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Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
Peptidoglycan Synthesis
Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan biosynthesis begins in...


