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螺旋烯A合成酶:来自Aspergillus luchuensis的一种循环酶,形成一个螺旋四环二烯

Zheng-Yu Huang¹, Kizerbo A Taizoumbe², Chengqin Liang³

¹State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China.

Angewandte Chemie (International ed. in English)

|November 14, 2023

概括

研究人员发现了一种新的二二碳化合物, spiroluchuene A,来自Aspergillus luchuensis. 通过先进的计算和实验方法阐明了其独特的螺旋环结构和生物合成.

科学领域:

自然产品化学自然产品化学
酶学是一种酶学.
计算化学计算化学

背景情况:

迪特尔合成酶是自然产品生物合成中的关键酶.
阿斯珀吉勒斯种类是众所周知的各种二次代谢产物的生产者.
新的化学支架为药物发现提供了机会.

研究的目的:

为了识别和表征来自阿斯伯吉勒斯luchuensis的新型二二烯合成酶.
阐明一种新的二烯碳化合物的生物合成途径和机制.
探索由真菌衍生的二烯的结构多样性和潜在应用.

主要方法:

酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS). 酶识别和表征 (AlTS).
同位素标记实验. 同位素标记实验.
密度函数理论 (DFT) 计算和元动力学模拟.
位点定向的突变发生和酶变异的分析.

主要成果:

从阿斯伯吉勒斯luchuensis催化 spiroluchuene A 形成的 ALTS 的鉴定.
对新型螺旋环二烯的循环化机制的阐明.
生物合成中间体luchudiene和衍生物spiroluchuene的捕获和结构特征 B.
证明了luchudiene与germacrenes的关系以及Cope重组的潜力.

结论:

AlTS产生一种独特的二二碳化合物,具有螺旋环系统.
生物合成涉及复杂的循环机制,通过结合实验和计算方法来阐明.
酶工程可以产生新的中间体,扩大二类的化学空间.

关键词:

生物合成生物合成酶的机制酶的机制同位素同位素是同位素.螺旋环是螺旋环中的一个.类物质是指类物质.

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