碳水化合物结合模块对一种真菌粉活性性多糖体单氧化酶的影响
Nan Zhang1, Jianhua Yang1,2, Zhimin Li3
1Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin 300308, P. R. China.
Journal of agricultural and food chemistry
|November 14, 2023
概括
碳水化合物结合模块 (CBMs) 显著增强了粉活性酸多糖单氧酶 (LPMOs). 这些CBM改善了基质结合和过氧化的产生,提高了氧化产品的产量,以更好地降解粉.
科学领域:
- 生物化学和酶学 生物化学和酶学
- 碳水化合物的化学成分
- 蛋白质工程是一种蛋白质工程.
背景情况:
- 非催化碳水化合物结合模块 (CBMs) 对于性多糖单氧酶 (LPMOs) 功能至关重要.
- 在粉活性AA13LPMOs中CBM的作用仍未得到充分研究.
研究的目的:
- 系统地研究四种新型CBM对粉活性LPMO (MtLPMO) 的影响.
- 评估CBM对基质结合,H2O2产生,氧化产品产量和与其他酶的协同降解的影响.
主要方法:
- 四个MtLPMO-CBM模拟器的建造和描述.
- 测试基质与各种粉基质的结合亲和力.
- 测量H2O2生产活动.
- 在存在α-amylase和glucoamylase的情况下,定量氧化产品产量和减少糖生成.
主要成果:
- MtLPMO-CnCBM对各种粉类型表现出最高的基质结合亲和力.
- MtLPMO-PvCBM和MtLPMO-CnCBM显著提高了H2O2的产量 (分别是4.6倍和3.6倍).
- 经CBM修改的LPMOs产生了显著更高的氧化产品,其中米洛白,玉米粉和粉分解的显著增加.
- 当与α-amylase结合时,MtLPMO-AcCBM改善了减少糖的产量.
结论:
- 在调节粉活性AA13LPMOs的活性方面,CBM起着至关重要的作用.
- 通过CBM增强基质结合和H2O2产生,提高了粉降解效率.
- 这些发现突出了CBM工程在优化LPMO在生物质转换中的性能方面的潜力.
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