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相关概念视频

X-Inactivation01:58

X-Inactivation

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The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Leveling Effect01:29

Leveling Effect

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In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the...
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Natural Selection and Adaptation01:15

Natural Selection and Adaptation

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Natural selection, a fundamental concept in evolutionary biology, is the mechanism by which evolution is driven, favoring organisms that are best adapted to their environments. This process enhances their chances of survival and reproduction. Adaptation, a key outcome of this process, involves genetic modifications that optimize an organism's functionality under specific environmental challenges, such as extreme cold or thinner air at high altitudes.
Beyond physical adaptations,...
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相关实验视频

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Primer-Free Aptamer Selection Using A Random DNA Library
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通过并行图书馆选发现功能增强的XNA Aptamers

Adriana Lozoya-Colinas1, Yutong Yu1, John C Chaput1,2,3,4

  • 1Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California 92697-3958, United States.

Journal of the American Chemical Society
|November 14, 2023
PubMed
概括

研究人员开发了一种新的单一选方法来发现核酸体. 这种方法加快了对治疗点的高亲和性体的识别,包括SARS-CoV-2蛋白质.

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科学领域:

  • 生物化学
  • 分子生物学
  • 药物发现

背景情况:

  • 核酸体对于向与疾病相关的蛋白质至关重要.
  • 传统的体外进化方法 (SELEX) 耗时,限制了序列和目标组合的探索.

研究的目的:

  • 开发一种快速的,单一的选方法来发现体.
  • 为了加快对治疗标的高亲和度aptamers的识别.

主要方法:

  • 一次性查方法,使用功能增强型化学物质.
  • 针对SARS-CoV-2 S1蛋白受体结合域的三聚体的亲和选择.
  • 选择的体的生物化学特征.

主要成果:

  • 使用新方法证明了新发现的体.
  • 鉴定出具有与常规SELEX产生的体相比较的结合亲缘关系的体.
  • 建立了一种高度可并行的aptamer发现方法.

结论:

  • 一次性查方法显著加快了体的发现.
  • 这种方法为更高效地发现治疗性胺提供了可行的途径.
  • 这种方法可以同时探索药物开发的各种化学产品.