相关实验视频

Updated: Jul 11, 2025

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

从单个小鼠卵巢中分离单个细胞,用于流细胞计和功能分析

Enitome E Bafor¹, Toni Martin¹, Jeffrey Carrell²

¹Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.

|November 14, 2023

概括

这项研究详细介绍了一种经过验证的工作流程,用于隔离可行的单个小鼠卵巢细胞,这对于免疫细胞研究至关重要. 该协议增强了单细胞分析和体外测定,而无需将样本组合在一起.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

卵巢细胞隔离通常需要组合样本,限制个人分析.
了解卵巢免疫细胞种群对于生殖健康研究至关重要.

研究的目的:

提出一个经过验证的工作流程,以从单个卵巢中分离出可活的单个小鼠卵巢细胞.
为了实现下游应用,如流细胞计和体外检测.
为了最大限度地提高免疫细胞的产量,包括T细胞子集.

主要方法:

详细的雌性分期,卵巢采集和酶分离.
优化了卵巢细胞染色协议,用于多参数分析.
对于适用于各种分析的单细胞悬浮液的工作流验证.

主要成果:

从一只小鼠中成功分离出足够多的可活单卵巢细胞.
单细胞悬浮剂在流细胞计和体外检测中已被证明是有用的.
免疫细胞的最大化隔离效率,包括特定的T细胞子集.

结论:

本工作流提供了一个强大的方法,用于单个小鼠卵巢单细胞隔离.
该协议有助于对卵巢免疫微环境进行详细分析.
该方法支持繁殖免疫学和细胞生物学中的多种下游应用.

关键词:

细胞隔离细胞的隔离.流动细胞计量 (Flow Cytometry) 是一种流动细胞计.一个单细胞单细胞.

更多相关视频

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Published on: May 2, 2018

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Published on: November 4, 2021

相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.