宿主基因表达的调节由J类偏amyxovirus进行
Elizabeth R Wrobel1, Jared Jackson1, Mathew Abraham1
1Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
PloS one
|November 14, 2023
概括
艾滋病病毒 (JPV) 感染改变了小鼠纤维细胞中的宿主基因表达,从早期的干扰素反应转移到晚些时候的抗原处理. 这揭示了关键的免疫路径,涉及到Jeilongvirus的发病过程.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 帕拉米克索病毒会引起人类和动物的重大疾病.
- 济龙病毒是Paramyxoviridae的一个属,在野生动物中越来越多地发现.
- 济龙病毒对宿主基因表达的影响在很大程度上是未知的.
研究的目的:
- 为了研究宿主基因表达在小鼠纤维细胞中感染J类偏amyxovirus (JPV) 后的变化.
- 为了确定由Jeilongvirus感染调节的特定免疫路径.
主要方法:
- 鼠标纤维细胞被JPV感染,RNA在多个时间点 (2-48小时感染后) 被测序.
- 使用Tophat2-Cufflinks-Cuffdiff管道进行了差异基因表达分析.
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 鉴定出了大量的差异表达基因 (DEG),从2hpi的11个增加到48hpi的1837个.
- 早期的JPV感染主要影响干扰素反应基因.
- 后期阶段显示,基因转向参与抗原处理,呈现和免疫反应途径的基因.
- qRT-PCR证实了Rtp4,Ifit3,Mx2,Stat2的上调和G0s2.2的下调.
结论:
- JPV感染以时间依赖的方式对宿主基因表达产生深远影响.
- 这项研究阐明了宿主免疫反应机制对抗Jeilongvirus.
- 这些发现为制定针对潜在的龙病毒相关疾病的策略提供了洞察力.
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