线索与奖励编码的底侧杏仁体投射到核
Yi He1, Yanhua H Huang2, Oliver M Schlüter1
1Departments of Neuroscience, University of Pittsburgh, Pittsburgh, United States.
eLife
|November 14, 2023
概括
两个截然不同的底侧杏仁体 (BLA) 神经元类型投射到核 (NAc). Ppp1r1b子投影,而不是rspo2,选择性地驱动提示诱导的寻找药物和复发物质使用障碍.
科学领域:
- 神经科学是一个神经科学.
- 成研究 研究成研究
- 分子精神病学分子精神病学
背景情况:
- 药物使用障碍包括通过药物强化 (无条件刺激,US) 和与药物相关的线索 (有条件刺激,CS).
- 底侧杏仁体 (BLA) 在US和CS介导的药物反应中发挥作用.
- 在BLA中,不同的神经元群体可能会对这些过程有不同的贡献.
研究的目的:
- 研究两种基因定义的BLA神经元类型 (Rspo2-和Ppp1r1b-表达) 在调解药物强化和提示诱导的药物寻找中的不同作用.
- 为了阐明核心聚合体 (NAc) 中的US和CS驱动行为背后的电路机制.
主要方法:
- 在小鼠中利用光遗传刺激和化学遗传学.
- 研究了内自我刺激 (ICSS) 和暗示诱导的可卡因寻找.
- 从不同的BLA神经元类型对NAc的预测和它们与多巴胺受体表达神经元的连接进行了检查.
主要成果:
- 无论是rspo2还是pp1r1b,BLA神经元都向NAc投射,并与多巴胺D1和D2受体神经元连接.
- 在NAc支持的ICSS中刺激Rspo2或Ppp1r1b终端.
- 只有表达Ppp1r1b的BLA神经元可以调解提示诱导的ICSS寻找.
- 调节Ppp1r1b-NAc通路,但不是RSpo2-NAc,改变了诱导可卡因寻求戒断后的提示.
结论:
- 对NAc的Ppp1r1b表达BLA亚投射选择性地编码受条件刺激 (CS) 介导的奖励和药物强化.
- 虽然这两种BLA亚投射都对无条件刺激 (US) 中介反应有所贡献,但Ppp1r1b神经元对于提示驱动的药物寻找至关重要.
- 这些发现提供了对区分和潜在操纵成中药与暗示诱导的行为的见解.
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