在化学反应敏捷的合成细胞中以光驱动的膜组装,形状变化和组织形成
Youngjun Lee1, Alessandro Fracassi1, Neal K Devaraj1
1Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
Journal of the American Chemical Society
|November 14, 2023
概括
研究人员使用光敏感脂质开发出动态合成细胞. 这些人造细胞可以组装成复杂的组织,模仿生物功能,并提供早期生命演变的见解.
科学领域:
- 仿生化学
- 合成生物学
- 材料科学
背景情况:
- 生物系统通过受控的组装动态表现出复杂的组织.
- 一个关键的问题是, 合成细胞是否可以通过脱离平衡来实现类似的复杂性.
- 了解能量驱动组装对于创造功能性人工生命至关重要.
研究的目的:
- 设计具有复杂自组合和功能能力的动态合成细胞.
- 研究外部刺激 (光,化学物质) 在控制合成细胞行为的作用.
- 探索从这些动态细胞中创造功能合成组织的潜力.
主要方法:
- 使用人工的,对光和化学反应的脂质组装合成细胞.
- 使用光辐射诱导囊泡形成和结构重塑.
- 包括多价值聚合物来触发囊泡交叉链接和组织形成.
主要成果:
- 乱的脂质聚合物在照射时自发地形成巨大的细胞状囊泡,当光线被移除时会逆转.
- 改造的合成细胞膜与构建块相互作用, 防止聚合和改变细胞膜组成.
- 光引起的形状变化 (球变成棒) 模仿了依赖能量的生物功能.
- 从脂质聚合物中形成功能合成组织.
结论:
- 动态合成细胞可以被设计成表现出复杂的,依赖能源的行为.
- 光和化学刺激提供精确的控制合成细胞组装成等级结构.
- 这个系统为了解早期原细胞如何利用能量进行协调组装提供了一个模型.
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