一个全细胞生物转化的新策略,使得同时产生l-酸和辅酶再生成为可能
Huidong Xu1, Qianqian Cheng1, Yangyu Qiu1
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|November 14, 2023
概括
一种新的l-Phenyllactic acid (l-PLA) 生物合成方法使用两种酶进行经济高效的生产. 这种环保工艺提高了l-PLA的产量并简化了生产,为有机酸合成提供了一个多功能平台.
科学领域:
- 生物技术是生物技术.
- 生物催化剂是一种生物催化剂.
- 代谢工程是代谢工程.
背景情况:
- l-Phenyllactic acid (l-PLA) 是一种有价值的有机酸,具有针对食物传播病原体的显著抗微生物特性.
- 目前用于l-PLA生物合成的方法可能是昂贵和复杂的,需要更高效和可持续的方法.
研究的目的:
- 开发一种用于l-PLA生物合成的新,经济高效,环保的全细胞生物转化系统.
- 为了设计一种简化的酶级联,只使用两个异质酶,以高效地生产l-PLA和共酶再生.
主要方法:
- 设计了一种全细胞生物转化系统,利用氨酸脱酶 (PheDH) 和l-氧化酸脱酶 (l-HicDH).
- 在两种酶系统中,用于l-PLA生产和综合辅酶 (NAD+/NADH) 再生的级联催化.
- 优化酶表达和生物合成参数,以最大限度地提高l-PLA产量.
主要成果:
- 获得了2.53 ± 0.07 g/L的初始l-PLA产量.
- 成功地将l-PLA产量提高到4.68 ± 0.04 g/L,优化后的产率为64.54 ± 0.29%.
- 通过改变基质和酶组合,证明了该系统在生产其他有机酸的多功能性.
结论:
- 这种新的两种酶系统为l-PLA生物合成提供了一种简化且具有成本效益的方法.
- 这一战略提高了有机酸生产的可持续性,并为生物技术应用提供了一个多功能平台.
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