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Updated: Jul 11, 2025

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适应RiPCA用于mRNA-蛋白相互作用的活细胞检测

Dalia M Soueid1, Amanda L Garner1

  • 1Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, United States.

Biochemistry
|November 14, 2023
PubMed
概括

RNA结合蛋白 (RBPs) 通过与细胞RNA相互作用来调节基因表达. 这项研究将RNA相互作用与蛋白介导补充试验 (RiPCA) 进行调整,以检测和选活细胞中这些关键的mRNA-蛋白相互作用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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