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哺乳动物细胞中蛋白质的深度突变扫描

Stefanie Maes1, Nick Deploey2, Frank Peelman2

  • 1VIB Center for Medical Biotechnology (CMB), Technologiepark-Zwijnaarde 75, 9052 Ghent, Belgium; Department of Biochemistry and Microbiology, Ghent University, Technologiepark-Zwijnaarde 75, 9052 Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Technologiepark-Zwijnaarde 75, 9052 Ghent, Belgium.

Cell reports methods
|November 14, 2023
PubMed
概括

哺乳动物细胞中的深度突变扫描使蛋白质变异的功能分析成为可能. 本指南详细介绍了解释遗传变异的实验方法,帮助疾病研究.

关键词:
CP: 生物技术 生物技术科普:遗传学 在CP:遗传学.深度突变扫描 (deep mutational scanning) 是一种对突变进行深度扫描的方法.基因组编辑 基因组编辑哺乳动物哺乳动物和突变发生的原因是和突变.结构/功能分析分析.不知重要性的变体.

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