通过mTOR信号通路,METTL3通过暴露于高血糖的胎盘调节葡萄糖载体表达
Jie Ning1,2,3, Jing Huai1,2,3, Shuxian Wang1,2,3
1Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing 100034, China.
Chinese medical journal
|November 14, 2023
概括
怀孕期间的高血糖会对热囊细胞中的甲基转移酶类3 (METTL3) 进行上调,然后通过mTOR信号控制葡萄糖转运体 (GLUTs),破坏营养物质的运输.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞代谢的细胞代谢.
背景情况:
- 怀孕期高血糖症 (HIP) 与胎盘葡萄糖载体 (GLUTs) 表达的改变有关.
- 高葡萄糖环境对HIP胎盘GLUTs的确切影响尚不清楚.
研究的目的:
- 调查高血糖症对胎盘GLUTs表达和潜在的调节机制的影响.
- 在这个过程中,探索哺乳动物中拉巴胺素 (mTOR) 和甲基转移酶类3 (METTL3) 的标的作用.
主要方法:
- 免疫组织化学用于评估胎盘mTOR和GLUTs (GLUT1,GLUT3,GLUT4) 在正常怀孕和2型糖尿病 (T2DM) 中的表达.
- 贝沃细胞暴露在不同的葡萄糖度下,并采用了mTOR通路调制 (MHY1485/拉帕米辛,siRNA).
- 此外,还研究了METTL3在高血糖反应中的作用.
主要成果:
- 在T2DM怀孕中,胎盘mTOR,p-mTOR和GLUT1的上调.
- 超血糖症增加了mTOR活性,并提高了BeWo细胞中的GLUTs表达和GLUT1细胞膜转位.
- 高葡萄糖对METTL3产生上调,其调节影响了GLUTs表达和mTOR活动,而METTL3影响了mTOR信号传递.
结论:
- 高葡萄糖诱导热囊细胞中METTL3的上调,后者通过mTOR信号调节GLUTs的表达.
- 这一途径有助于在怀孕期间高血糖症的妇女中破坏营养物质的运输.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Insulin: The Receptor and Signaling Pathways
1.2K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
1.2K
Diabetes Mellitus: Type 2 and Gestational
2.5K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
2.5K
Glucose Transporters
22.8K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.8K


