相关实验视频
Updated: Jul 11, 2025

06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
14.9K
在单个短基堆叠单链RNA分子中的电荷运输
Subrata Chandra1, Ajoke Williams1, Farkhad Maksudov1
1Department of Chemistry, University of Massachusetts, Lowell, 01854, USA.
Scientific reports
|November 14, 2023
概括
单链RNA表现出可测量的电导率,这是由于延长的基堆叠,与DNA不同. 这一发现促进了对电子和生物传感器生物分子中电荷传输的理解.
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 纳米技术纳米技术
背景情况:
- 生物分子中的电荷传输对于生物功能和生物传感器等技术应用至关重要.
- 了解RNA的电荷传输特性是非常重要的,因为它具有生物医学意义,但仍然不太了解.
研究的目的:
- 测量和理解单链RNA (ssRNA) 序列的电导率.
- 与DNA相比,研究影响RNA中电荷传输 (CT) 的结构和电子因素.
主要方法:
- 利用扫描道显微镜辅助的分子断裂连接方法来确定单分子导电.
- 采用循环二元化 (CD) 光谱和分子动力学 (MD) 模拟来分析分子构造.
- 应用计算分子建模和机器学习用于数据解释.
主要成果:
- 具有基地堆叠能力的ssRNA序列显示出可测量的单分子导电性 (约为10-6S).
- 相当长的单链DNA (ssDNA) 序列表现出没有特征的导电性组图.
- 识别了带有基堆叠的扩展ssRNA形状,与折叠的ssDNA形状形成鲜明对比.
结论:
- 在RNA中可测量的电荷传输与具有非局部化HOMO能量水平的延伸,基层堆叠稳定形状有关.
- 在RNA中,有效的电荷传输是由基堆叠路径促进的,与DNA形成封闭结构的倾向不同.
相关概念视频
Nucleic Acid Structure
6.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
6.2K
RNA Stability
33.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.6K
RNA Structure
71.5K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.5K
Nucleic Acids
44.3K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
44.3K
Nucleic acids
163.3K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
163.3K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K

