使用纳米孔感应和编码分子探针进行病毒抗原和RNA的多重检测
Ren Ren1,2, Shenglin Cai3,4, Xiaona Fang5
1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
Nature communications
|November 14, 2023
概括
这项研究引入了一种新的纳米孔传感方法,使用DNA探针检测SARS-CoV-2蛋白质和RNA突变. 该技术能够准确地从患者样本中直接识别像Omicron这样的病毒变体,而无需进行测序.
科学领域:
- 纳米技术 纳米技术
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 准确和快速检测SARS-CoV-2对于控制流行病至关重要.
- 现有的方法通常需要复杂的样本处理或核酸测序.
- 需要一种多功能诊断工具,能够识别病毒蛋白和遗传突变.
研究的目的:
- 开发一个单分子纳米孔感应平台,同时检测SARS-CoV-2抗原和RNA.
- 证明识别特定病毒突变和区分主要SARS-CoV-2变种的能力.
- 建立一个敏感和选择性的诊断策略,适应各种病毒标.
主要方法:
- 利用单分子纳米孔传感,与位置编码的DNA分子探针集成.
- 开发了用于同时检测病毒蛋白 (尖峰和核囊) 和RNA片段的特定化学物质.
- 对未经加工的人体唾液和鼻子/喉抽样进行了测试.
主要成果:
- 在未经加工的人类唾液中成功检测到SARS-CoV-2尖峰 (S) 和核囊 (N) 蛋白质.
- 在患者样本的RNA片段中确定了关键的SARS-CoV-2突变 (D614G,G446S,Y144del).
- 在没有测序的单个测量中区分SARS-CoV-2野生型,Alpha,Delta和Omicron变体.
结论:
- 开发的纳米孔感应策略为SARS-CoV-2检测提供了高灵敏度和选择性.
- 这种方法可以直接从临床样本中快速识别病毒变异和突变.
- 这种可适应的传感平台有可能在传染病诊断中得到更广泛的应用.
更多相关视频
07:24Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
Published on: October 29, 2020
2.8K
11:00High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
12.0K
相关概念视频
03:21RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K