糖菌对抗热应激的生存需要CCT和细胞壁完整性通路之间的通信
Ankita Dube1, Dileep Pullepu2, M Anaul Kabir3
1Department of Biochemistry, Indian Institute of Sciences, Bangalore, India.
Biologia futura
|November 14, 2023
概括
在酵母中,沙佩罗宁CCT7基因的突变破坏了蛋白质折叠和细胞壁完整性. 过度表达细胞壁完整性通路基因拯救了这些缺陷,揭示了CCT和细胞壁维护之间的联系.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 在酵母中蛋白质折叠必不可少的Chaperonin TRiC/CCT复合体,由CCT1-8基因编码的16个子单元组成.
- 在Cct7p中发生的一种特定突变 (G412E) 会导致基质错折和细胞缺陷.
研究的目的:
- 为了研究Cct7p G412E突变的功能后果.
- 探索酵母菌中CCT功能和细胞壁完整性之间的关系.
主要方法:
- 一种Cct7p突变酵母菌株 (G412E) 的表征.
- 对热冲击和细胞壁压力因素的敏感度的评估.
- 分析细胞壁完整性 (CWI) 途径和MAPK信号通路中的基因表达.
- 补充研究涉及基因过度表达.
主要成果:
- 突变Cct7p G412E对热量和细胞壁压力因素表现出敏感性,表明蛋白质折叠和细胞壁维护受损.
- 来自MAPK通路的PKC1和SLT2基因过度表达挽救了生长和细胞分裂缺陷.
- 关键的CWI通路基因 (SED1,GFA1,PIR1,RIM21) 在突变者中被下调.
结论:
- Cct7p G412E突变通过影响蛋白质折叠和细胞壁完整性,破坏细胞平衡.
- 在 chaperonin TRiC/CCT 复合体和酵母细胞壁完整性通路之间存在显著的功能相互作用.
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