封装Farnesol的基托桑纳米颗粒在体内对Candida albicans进行了评估,对Candida albicans进行了评估
Adelaide Fernandes Costa1, Jacqueline Teixeira da Silva2, Juliana Assis Martins2
1Biotechnology, Institute of Tropical Pathology and Public Health, Universidade Federal de Goiás, Goiânia, GO, 74605-050, Brazil. adelaidefernandes@ufg.br.
概括
含有法内索尔的奇托桑纳米颗粒 (NF) 通过减少真菌负荷并防止和生物膜形成,有效抑制白菌. 这些纳米粒子在体内没有表现出毒性,为抗真菌治疗提供了有希望的替代方案.
科学领域:
- 纳米技术 纳米技术
- 菌群学 菌群学是指菌群学的学科.
- 药理学 药理学是指药理学的学科.
背景情况:
- 法尔内索尔是一种天然的精油,具有抗微生物特性.
- 基托桑纳米颗粒增强了法内索尔的生物可用性和抗真菌功效.
- 针对和生物膜的形成对于对抗真菌感染至关重要.
研究的目的:
- 开发和描述Farnesol装载的奇托桑纳米粒子 (NF).
- 评估NF的体内毒性.
- 评估NF对Candida albicans的抗真菌作用.
主要方法:
- 对于纳米粒子制备的离子凝方法.
- 物理化学特征 (大小,泽塔潜力,形态,稳定性).
- 在Galleria mellonella和小鼠模型中进行体内毒性评估.
- 对G. mellonella和小鼠性阴道角膜炎模型中的C. albicans的抗真菌功效评估.
主要成果:
- NF显示的粒子直径<200nm,单分散分布,正泽塔潜力和稳定性.
- 在测试剂量时,没有观察到Galleria mellonella显著的毒性.
- NF治疗显著降低了真菌负荷,抑制了和生物膜的形成,并增加了感染的G. mellonella的生存率.
- 在小鼠VVC模型中,NF降低了炎症,真菌负担和细胞生长.
结论:
- 含法内索尔的奇托桑纳米颗粒是稳定的,无毒的,并且具有强大的 in vivo 抗真菌活性.
- NF有效地抑制了Candida albicans,包括海法和生物膜的发展.
- 这些纳米颗粒代表了管理C. albicans感染的有希望的替代方案.
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