13个亚洲Justicia (Acanthaceae) 种的完整塑基因组结构:比较基因组学和家族遗传学分析
Zhengyang Niu1,2, Zheli Lin1,3, Yi Tong4
1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
BMC plant biology
|November 14, 2023
概括
这项研究对12种亚洲 Justicia 植物的叶绿体基因组进行了测序,揭示了 Justicia 是多体的,并根据基因组和形态学证据确定 Justicia grossa 是一个独特的新属.
科学领域:
- 植物学 植物学
- 基因组学就是基因组学.
- 人类遗传学 是一个学科.
背景情况:
- Justicia L. 是Acanthaceae中最大的属,分布广泛,具有重要的药用/装饰价值.
- 之前对Justicia的遗传学研究仅限于亚洲的样本采集,阻碍了对该地区的基因组结构和关系的理解.
- 尽管基因研究很重要,但对Justicia的基因研究很少.
研究的目的:
- 提高对亚洲 Justicia 植物的基因组结构和物种间关系的理解.
- 使用完整的叶绿体基因组进行比较基因组学和遗传学分析.
- 调查Justica物种的遗传位置,特别是来自亚洲的物种.
主要方法:
- 完整的叶绿体 (cp) 的测序. 来自12个亚洲 Justicia 植物的基因组.
- 进行比较基因组学分析,结合先前发表的cp. 这是Justicia leptostachya的基因组.
- 遗传学分析以解决 Justicia 属内的关系.
主要成果:
- 所有测序的cp. 基因组显示出典型的四部分结构,没有重新排列或基因丢失,范围从148,374到151,739 bp.
- Justicia grossa表现出明显的基因组特征,包括更短的ndhF和ycf1基因在反向重复 (IR) 和更高的SSR和分散重复的丰度,这表明它不同于其他 Justicia物种.
- 遗传学分析证实Justicia是一个多种族群,J. grossa属于Tetramerinae亚族,其他成员属于Justiciinae亚族,支持将J. grossa重新归类为一个新属. 鉴定出ycf1基因是Justicia属的潜在DNA条形码.
- 发现Justicia的进化历史与其形态演变相一致.
结论:
- 这项研究首次阐明了亚洲 Justicia 植物中整个塑体的基本特征.
- 这项研究揭示了亚洲Justicia内部的跨物种关系,揭示了Justicia作为一个多种类的属.
- 形态和分子证据强烈支持将Justicia grossa认定为一个新属,与其他Justicia物种不同.
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