网格2相互作用蛋白是一种与结直肠癌免疫透相关的潜在生物标志物
Jiajing Zhao1, Jiazheng Quan2, Weilin Chen3
1Department of General Surgery, the First Affiliated Hospital of Shantou University Medical College, Shantou, 515000, China.
European journal of medical research
|November 15, 2023
概括
在结肠直肠癌 (CRC) 中,GRID2IP的调节升高,与免疫细胞透率降低和患者预后较差相关. 这表明GRID2IP可能作为CRC的预后生物标志物和治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 结肠直肠癌 (CRC) 是一个重大的全球健康挑战,其晚期的生存率不令人满意.
- 网格2相互作用蛋白 (GRID2IP) 是一个后突触支架蛋白,已知在神经系统信号传递中的作用以及与其他癌症的联系.
- 了解GRID2IP在CRC中的作用对于改善患者的治疗结果至关重要.
研究的目的:
- 研究GRID2IP表达与结直肠癌的发生和预后之间的关系.
- 探索GRID2IP在CRC中的功能背后的分子机制,包括它对免疫透和信号通路的影响.
- 评估GRID2IP作为CRC的潜在预后生物标志物和治疗点.
主要方法:
- 在癌症基因组图谱 (TCGA) 数据库中的GRID2IP表达的分析和在细胞系 (HT29,SW480) 中的验证,使用qPCR和Western Blot.
- 不同基因表达分析 (DESeq2),基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
- 基因组丰富分析 (GSEA),单样本基因组丰富分析 (ssGSEA),卡普兰-梅尔生存分析,考克斯回归建模和单细胞分析 (TISCH数据库).
主要成果:
- 发现GRID2IP在结肠直肠癌 (CRC) 患者中被上调,与免疫细胞透率降低和免疫评分降低有关.
- 在各种临床亚组中,高GRID2IP表达与整体存活率 (OS),疾病特异性存活率 (DSS) 和无进展间隔 (PFI) 差相关.
- 单细胞分析表明GRID2IP在免疫细胞,肌纤维细胞和癌细胞中的表达,高表达与降低对凝胺化疗的敏感性有关.
结论:
- 在CRC中GRID2IP的高表达促进了与瘤相关的免疫细胞透,导致不良的临床结果.
- GRID2IP可以作为一个有价值的生物标志物来预测CRC预后.
- GRID2IP代表了结直肠癌治疗的潜在治疗标.
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