辅酶反应因子微调素生物合成,以应对竹子中的机械曲
Wenjia Wang1, Yigang Li1, Changyang Cai1
1Basic Forestry and Proteomics Center (BFPC), College of Forestry, Haixia Institute for Science and Technology, Fujian Agriculture and Forestry University, 350002, Fujian, China.
The New phytologist
|November 15, 2023
概括
植物激素辅酶直接控制竹子中的红素生物合成,在曲过程中影响其机械性能. 这项研究揭示了素反应因子 (ARF) 3和ARF6如何调节关键的素基因,增强竹子的作用.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 素对于植物的机械强度至关重要,特别是在曲应力下.
- 植物激素auxin调节各种植物发育过程.
- 关联奥克辛与竹子曲诱导的红素生物合成的精确机制在很大程度上是未知的.
研究的目的:
- 为了阐明auxin在调节Moso竹 (Phyllostachys edulis) 在曲负荷下的素生物合成中的作用.
- 为了确定与素相关的特定因素及其基因参与竹木化.
主要方法:
- 转录组分析和权重基因共表达网络分析 (WGCNA) 用于确定基因表达相关性.
- 在曲应力下对奥素和红素分布模式的分析.
- ChIP-seq (染色体免疫沉降测序) 测定ARF蛋白直接结合到向基因促进体.
- 使用转基因竹子对ARF3和ARF6.6的改变表达的功能验证.
主要成果:
- 在曲反应中,auxin和lignin呈现不对称的分布.
- 辅酶反应因子 (ARF) 3和ARF6的表达与素生物合成基因有很强的相关性.
- ARF3和ARF6直接结合并激活关键素通路基因 (4CL3,4CL7,4CL9,CCoAOMT2) 的促进剂,结合效率取决于辅酶水平.
- 改变ARF3和ARF6表达显著影响了转基因竹子中的红素积累.
结论:
- 辅酶信号直接通过ARF3和ARF6.6调节竹子中的红素生物合成基因.
- 这种机制对于调节素含量至关重要,可能有助于竹子的变形稳定性.
- 这一发现为植物中木质化的遗传调节提供了新的见解.
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