Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genetic Variability, Trait Association, and Multi-Trait Selection of New Indeterminate Tomato Genotypes Under Protected Cultivation.

Plants (Basel, Switzerland)·2026
Same author

Hydrophyte root microbiome: a novel reservoir of plant growth-promoting bacteria for enhancing lettuce (Lactuca sativa L.) growth and nutritional quality under soilless cultivation.

World journal of microbiology & biotechnology·2026
Same author

Genetic evaluation of diverse sex expression to improve earliness and yield in bitter gourd (Momordica charantia L.) hybrids.

BMC plant biology·2026
Same author

Temperature stress induced anatomical and histological alterations in reproductive organs of Capsicum annuum L.

Protoplasma·2026
Same author

Spectral characterization and severity assessment of rice brown planthopper damage using multivariate models.

Scientific reports·2026
Same author

Histological insights in to cryopreservation of <i>Rubia cordifolia</i> L. using encapsulation-dehydration.

3 Biotech·2026

相关实验视频

Updated: Jul 11, 2025

In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors
08:50

In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors

Published on: July 16, 2018

8.2K

简单的Luffa花粉冷保存协议:提高繁殖效率

Arvind Nagar1, Ravi Gowthami2, Amish Kumar Sureja3

  • 1Krishi Vigyan Kendra (KVK), Jhalawar, Agricultural University, Kota, India.

Frontiers in plant science
|November 15, 2023
PubMed
概括
此摘要是机器生成的。

一个新的冷保存协议有效地长期存储了Luffa花粉. 这种方法保持了花粉的生存能力,以改善作物,确保高发芽和受精率,与新鲜花粉相美.

关键词:
卢法航空公司 (Luffa Luffa) 是一个航空公司.化保存的方法化温度是化时间.长期储存是长期储存的方法.对于花粉来说,这是一个很好的选择.储存温度 储存温度 储存温度超低结度 超低结度 超低结度

更多相关视频

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.8K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

31.1K

相关实验视频

Last Updated: Jul 11, 2025

In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors
08:50

In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors

Published on: July 16, 2018

8.2K
Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

Published on: October 9, 2017

9.8K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

31.1K

科学领域:

  • 植物生殖生物学 植物生殖生物学
  • 结冷保存技术的使用方法.
  • 园艺科学 园艺科学

背景情况:

  • 长期储存花粉对于改善作物和保护遗传资源至关重要.
  • 现有的 Luffa 花粉存储方法有限,阻碍了高效的作物育种计划.
  • 了解花粉发芽和冷保存的最佳条件对于Luffa物种至关重要.

研究的目的:

  • 开发和优化四种Luffa物种的长期花粉存储协议.
  • 评估冷保存的Luffa花粉在改善作物的生存能力和有效性.
  • 建立一个简单,可重复的方法,用于冷保存Luffa花粉.

主要方法:

  • 确定了L. acutangula,L. cylindrica,L. echinata和L. graveolens的体外最佳花粉发芽介质.
  • 通过改变干燥时间和监测水分含量来研究冷保存.
  • 评估了花粉活力,发芽,卵子受精,以及196°C冷保存后的果实/种子组.

主要成果:

  • 最佳的发芽介质有所不同:Brewbaker和Kwack (BK) 在三种物种中含有10%的糖,而L. graveolens的糖为3%.
  • 冷保存在20-40分钟的干燥过程中产生了最佳结果,保持了14.04-18.55%的水分含量.
  • 在196°C冷保存的花粉在2个月内显示出高生存能力,在繁殖成功方面与新鲜花粉相美.

结论:

  • 成功开发了一种简单且可重复的冷保存协议,用于Luffa花粉.
  • 冷保存的Luffa花粉至少在两个月内保持高活力和功能.
  • 该协议促进了长期储存,并提高了Luffa作物改进的潜力.