从欧盟的角度来看:NGT引起的植物意外遗传变化 - - 它们对于全面的分子表征和风险评估的相关性
Franziska Koller1, Michael Cieslak1
1Fachstelle Gentechnik und Umwelt (FGU), Munich, Bavaria, Germany.
Frontiers in bioengineering and biotechnology
|November 15, 2023
概括
像CRISPR/Cas这样的新基因组技术 (NGT) 可以导致植物的意外遗传变化. 这些变化与传统育种不同,需要在市场批准之前对NGT植物进行强制性风险评估.
科学领域:
- 农业生物技术 农业生物技术
- 分子生物学分子生物学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 全球关于农业新基因组技术 (NGT) 监管的讨论正在进行中.
- 欧盟委员会正在为NGT工厂提出具体法规.
- 关键问题涉及NGT诱导的基因修饰的强制性风险评估.
研究的目的:
- 审查NGT引起的意外遗传变化,重点关注CRISPR/Cas应用.
- 为了比较NGT诱导的变化与常规育种中的非向性突变发生.
- 评估意外遗传变化对NGT植物风险评估的相关性.
主要方法:
- 专注于CRISPR/Cas和意外遗传修饰的文献综述.
- 对NGT与传统育种方法进行比较分析.
- 识别针对性DNA修饰的差异及其结果.
主要成果:
- NGT,特别是CRISPR/Cas,可以引入意想不到的遗传变化.
- 这些意想不到的变化不同于传统的突变发生的地点,背景和频率.
- 由此产生的基因组合和潜在的生物效应 (表型) 可能会带来风险.
结论:
- 来自NGT的意外遗传变化对于风险评估至关重要.
- NGT诱导的变化可能导致新的基因组合和表型,带来潜在的健康和环境风险.
- 强制性分子表征和风险评估对于用于释放或市场许可的NGT工厂至关重要.
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