设计一种针对COVID-19和结核病的双重疫苗
Carlyn Monèt Guthrie1,2, Xuejuan Tan1,2, Amber Cherry Meeker1,2
1Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, United States.
Frontiers in cellular and infection microbiology
|November 15, 2023
概括
一种表达SARS-CoV-2尖端蛋白融合的新型重组BCG疫苗诱导了小鼠对COVID-19和肺结核的强烈免疫反应,显示出作为双重疫苗的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 合成生物学 合成生物学
背景情况:
- 由SARS-CoV-2引起的COVID-19大流行仍然是一个全球卫生危机.
- 结核病是由Mycobacterium tuberculosis引起的,是主要的传染病.
- 再组合的Mycobacterium bovis Bacille Calmette-Guérin (BCG) 正在作为一种疫苗载体进行研究.
研究的目的:
- 开发一种表达M. tb Ag85A和SARS-CoV-2尖端RBD的融合蛋白的复合BCG菌株.
- 评估新型重组BCG菌株对SARS-CoV-2和M. tb.的免疫性.
主要方法:
- 使用合成生物学生成了一个重组的M. bovisBCG菌株 (AspikeRBD).
- 该菌株表达了M. tb Ag85A和SARS-CoV-2尖峰受体结合域 (RBD) 的融合蛋白.
- 评估了小鼠的免疫反应,包括T细胞激活和IgG产生.
主要成果:
- 该AspikeRBD菌株成功地表达了融合蛋白.
- AspikeRBD诱导了显著的SARS-CoV-2尖端特异性T细胞激活和IgG的产生.
- 与父母的BCG相比,AspikeRBD增强了M. tb Ag85A特定的T细胞和IgG反应.
结论:
- 再组合的M. bovis BCG菌株AspikeRBD显示出对SARS-CoV-2的强有力的免疫性.
- 此外,AspikeRBD菌株还能增强对M. tb.的免疫反应.
- 阿斯派克RBD显示出作为COVID-19和结核病的新型双重疫苗候选人的潜力.
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