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根据TCGA数据,识别与预后相关的lncRNA和肺状细胞癌中的细胞验证

Yishuang Cui¹, Yanan Wu¹, Mengshi Zhang¹

¹School of Public Health, North China University of Science and Technology, Department of Hebei Key Laboratory of Medical-Industrial Integration Precision Medicine, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, China.

Frontiers in oncology

|November 15, 2023

概括

这项研究确定了12个长非编码RNA (lncRNAs),在肺状细胞癌 (LUSC) 中具有预后相关性. 发现LINC00923和LINC01341抑制了瘤细胞的增殖和迁移,这表明它们作为LUSC生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

肺状细胞癌 (LUSC) 是一种主要的肺癌亚型,具有复杂的分子基础.
长非编码RNAs (lncRNAs) 越来越多地被认为是它们在癌症发育和进展中的作用.
确定预后生物标志物对于改善LUSC患者的治疗结果至关重要.

研究的目的:

确定长非编码RNA (lncRNAs) 在肺状细胞癌 (LUSC) 中具有预后意义.
研究候选 lncRNAs 在 LUSC 细胞增殖,入侵和迁移中的功能作用.

主要方法:

利用癌症基因组图谱 (TCGA) 存储库进行差异基因表达分析 (DESeq2).
执行单变体和多变体的考克斯回归来识别预后 lncRNAs.
在细胞系中使用核细胞质分离,反意义寡核酸 (ASO) 敲除,CCK-8,殖民地形成和跨井测定验证的候选 lncRNAs.

主要成果:

确定了12个候选 lncRNAs,包括7个下调和5个上调.
发现LINC00923和LINC01341主要是核局部化的.
击LINC00923和LINC01341显著抑制了LUSC细胞系 (H226,H1703) 的增殖,入侵和迁移.

结论:

确定了12个与LUSC的预后影响相关的lncRNAs.
LINC00923和LINC01341显示出作为预测LUSC患者结果的预后标志物的潜力.
这些lncRNAs在调节LUSC细胞攻击性方面发挥着功能性作用.

关键词:

现在我们来看一下 LINC0092323 的情况.联系我们 LINC0134141 在LUSC中使用LUSC.在TCGA中,TCGA就是TCGA.在cnRNA中.

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