在Streptococcus parasuis的一种整合性和结合性元素中出现了一种新的ISS1N-optrA载体转子体
Xingyang Dai1, Junjie Sun1, Wenbin Zhao1
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
The Journal of antimicrobial chemotherapy
|November 15, 2023
概括
在Streptococcus parasuis的新型转子体中发现了oxazolidinone耐药性基因 optrA. 这种转子子可以转移到其他细菌,可能传播耐药性.
科学领域:
- 细菌学 细菌学是一门学科.
- 遗传学 是一个遗传学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 氧利丁抗生素对于治疗由格拉姆阳性细菌引起的感染至关重要.
- 像OptraA这样的抗药基因的出现对公共健康构成重大威胁.
- 杆菌 (Streptococcus parasuis) 是一种重要的动物病原体,具有动物传播的潜力.
研究的目的:
- 为了研究氧化胺抗性基因 optrA 在Streptococcus parasuis 隔离物中的遗传环境和可转移性.
- 了解在细菌群体中OctrA传播的机制.
主要方法:
- 使用PCR和抗菌素敏感性测试对S. parasuis分离SFJ45的表征.
- 全基因组测序和生物信息分析以确定耐药性基因及其遗传背景.
- 结合实验用于评估 optrA 基因的可转移性.
- 脉冲场凝电泳 (PFGE) 和南方涂抹以确认基因转移.
主要成果:
- 在S. parasuis分离的SFJ45中,OptraA与其他多个耐药基因一起存在,表现出多药耐药 (MDR) 现型.
- 一个新型的8750 bp伪化合物转子体,ISS1N-hth-clb-4hp-optrA-2hp-ISS1N,携带 optrA,被确定.
- 这种转子被集成到一个集成和结合元件 (ICESpaSsuSFJ45),位于fda基因的下游.
- 从S. parasuis转移到Streptococcus suis的OctrA载体转体的结合转移在 (1.01 ± 3.12) × 10-7.的频率上被成功证明.
结论:
- ISS1N元素首次与 optrA 的传播有关.
- 在ICESpaSsuSFJ45中集成ISS1N-optrA转位子,促进了 optrA与其他抗菌素耐药性基因的共同选择.
- 这种机制促进了optrA从S. parasuis水平转移到更具有临床意义的细菌病原体.
更多相关视频
11:36Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Published on: September 23, 2017
16.0K
13:31Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Published on: October 31, 2014
14.0K
相关概念视频
Transposons
38
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
38
DNA-only Transposons
14.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.5K
Overview of Transposition and Recombination
15.6K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.6K
Mechanism of Conjugation
25
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
25
Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
LTR Retrotransposons
17.5K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.5K
