肺部有机体开发和疾病建模应用的最新进展
Ana I Vazquez-Armendariz1,2, Purushothama Rao Tata3,4,5
1University of Bonn, Transdisciplinary Research Area Life and Health, Organoid Biology, Life & Medical Sciences Institute, Bonn, Germany.
The Journal of clinical investigation
|November 15, 2023
概括
先进的肺器官现在更好地模仿人类的气道和气泡,改善疾病建模. 这些干细胞衍生模型为肺损伤,修复和复杂疾病提供了更深入的见解.
科学领域:
- 生物医学工程 生物医学工程
- 干细胞生物学 干细胞生物学
- 肺病学研究 肺病学研究
背景情况:
- 在过去的十年里,有机体模型在细胞,结构和功能复杂性方面取得了重大进展.
- 现有的肺器官平台越来越多地复制呼吸道和气泡的体内细胞结构.
研究的目的:
- 审查肺器官技术近期的进展.
- 讨论这些模型在研究肺部生物学和疾病发病过程中的应用.
- 概述肺器官发展的优点,局限性和未来方向.
主要方法:
- 开发用于干细胞隔离和培养的新方案.
- 从各种干细胞来源 (成人,胎儿,诱导多能干细胞) 生成先进的肺器官.
- 纳入非上皮细胞 (免疫,介质细胞,内皮细胞) 和基因编辑工具.
主要成果:
- 目前的肺器官模型紧密模仿气道和气膜细胞结构.
- 优化的协议使得研究肺损伤和修复中的细胞和分子机制成为可能.
- 包括多种细胞类型和基因编辑在体内增强了疾病建模的相关性.
结论:
- 肺器官技术显著提高了模拟肺部生物学和COPD,纤维化和感染等疾病的能力.
- 这些模型为体内研究提供了有价值的替代方案,用于调查复杂的肺病理和宿主微生物相互作用.
- 进一步的开发有望为更复杂,更准确的肺病建模提供希望.
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