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使用142个封闭基因组对Serratia marcescens复合物的质粒进行系统分析

Debora Satie Nagano¹, Itsuki Taniguchi¹, Tomoyuki Ono^1,2

¹Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka, 812-8582, Japan.

Microbial genomics

|November 15, 2023

概括

等离子体在Serratia marcescens复合体中对细菌基因组多样化有显著的贡献. 医院适应的血统和特定的环境显示出不同的等离子体配置,其中Klebsiella pneumoniae是等离子体交换的关键来源.

科学领域:

细菌基因组学是一种细菌基因组学.
等离子体生物学
微生物进化过程中的微生物.

背景情况:

质粒是细菌基因组多样化的关键驱动因素.
之前对基因组草案的分析表明,在Serratia marcescens复合体 (SMC) 中有显著的等离子体贡献.
从基因组草稿进行准确的等离子体分析是具有挑战性的.

研究的目的:

通过使用封闭的基因组序列,精确分析塞拉蒂亚马尔塞森综合体 (SMC) 内的等离体.
研究塑体在SMC基因组多样化和适应中的作用.
为了确定细菌物种内部和之间等离子体的来源和传播模式.

主要方法:

对Serratia marcescens复合体的142个封闭基因组进行分析,其中包括67个新测序的菌株.
132个等离子体 (1.9244.4 kb) 的识别和表征.
泛基因组分析以确定等离子体特异性和共享基因.
马什基于距离的聚类等离子体.
对等离子体GC含量的比较分析以及与其他物种的等离子体的同质性.

主要成果:

在77个SMC菌株中鉴定出132个等离子体;类2菌株 (医院适应) 含有更多的等离子体.
等离子体特异性基因占泛基因组的12.8%;抗微生物和重金属耐药性基因普遍存在.
等离子体团显示出来自宿主染色体的独特GC含量,表明最近获得.
在23个等离子体集群中,有17个是针对临床或非临床菌株的特异性,这表明了利基偏好.
在多个状体中发现了16个状体,这表明状体间的传播.
在其他物种中经常发现同类等离子体,特别是在Enterobacteriaceae家族中,Klebsiella pneumoniae被确定为等离子体交换的主要来源.

结论:

封闭基因组分析为等离子体种群提供了强大的洞察力.
等离子体在SMC基因组多样化中发挥着重要作用,在医院适应的血统中具有明显的模式.
克莱布西拉肺炎菌和其他肠杆菌菌是SMC获得等离子体的关键来源,突出了广泛的宿主范围和物种间的转移.

关键词:

GC内容GC内容的内容塞拉蒂亚马尔塞森斯 (Serratia marcescens) 综合体是一个复杂的植物群.关闭的基因组是封闭的.质粒的宿主范围范围.塑料质粒塑料质粒是什么?

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