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酵母Pcf11中的突变,这是对mRNA 3'端处理和转录终止至关重要的保存蛋白,引起环境应激反应

Joel H Graber¹, Derick Hoskinson², Huiyun Liu²

¹Mount Desert Island Biological Laboratory, Bar Harbor, ME 04609, USA.

|November 15, 2023

概括

这就是Pcf11蛋白质.

科学领域:

分子生物学分子生物学
基因表达规范基因表达规范
单核细胞转录单核细胞转录

背景情况:

在真核生物中,Pcf11蛋白对mRNA 3'末端处理和多化至关重要.
Pcf11还涉及基因循环,RNA聚合酶II (Pol II) 转录终止和mRNA输出.
一个保存的域 (氨基酸142-225) 的Saccharomyces cerevisiae Pcf11是很差的特征.

研究的目的:

为了研究保存的Pcf11域 (氨基酸142-225) 的功能.
确定该域在mRNA处理,转录终止和基因表达中的作用.
了解Pcf11如何调节基因表达和细胞反应.

主要方法:

对缺乏保存域或在Pol II CTD交互域中发生突变的Pcf11突变的分析.
全基因组的Pol II占用率分析.
全球基因表达分析.

主要成果:

对于mRNA 3'端处理或下游终结,Pcf11域不必.
这个域增强了Pol II的交互和在推动者附近使用终结器.
突变导致转录性变化,诱导一般应激反应,并导致细胞壁基因表达不平衡.
突变者表现出对咖啡因的敏感性,而没有激活细胞壁完整性通路.

结论:

Pcf11调节特定功能组的基因表达水平,而不依赖于它在mRNA 3'终端处理中的作用.
Pcf11域影响转录的启动和终止.
比以前理解的,Pcf11在调节基因表达和细胞应激反应方面发挥了更广泛的作用.

关键词:

环境应激反应环境应激反应在Pcf11中,Pcf11是什么?衰减减弱化是一种在mRNA 3′端处理过程中.转录终止转录终止

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