血小板碎片和细胞外囊泡的结构分析,这些碎片是由血小板在储存过程中产生的血小板
Silvia H De Paoli1, Mehulkumar Patel1,2, Oumsalama K Elhelu1
1Laboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD.
Blood advances
|November 15, 2023
概括
血小板储存损伤颗粒 (PSLP) 在储存期间增加,影响静血功能. 这些颗粒,包括脱粒的血小板和囊泡,在整个储存过程中都会释放出来,与激活相关的类型在早期出现,与细胞死亡相关的类型在晚些时候出现.
科学领域:
- 输血医学 输血医学
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
背景情况:
- 用于输血的血小板 (PLT) 在室温下 (RT) 有限的存储寿命长达7天.
- 由于储存损伤,缩PLT的储存会导致质量下降和血静功能受损.
- 膜状颗粒,称为血小板储存损伤颗粒 (PSLPs),是在PLT储存期间产生的.
研究的目的:
- 描述在血小板储存过程中产生的各种类型的膜性颗粒 (PSLPs).
- 分析PSLP度和成分在储存期间 (1-7天) 的变化.
- 调查不同PSLP种群的静血潜力和起源.
主要方法:
- 血小板单元被存储在室温下,并在存储1日,3日,5日,7日进行分析.
- 使用微分离心和糖密度梯度来隔离和分离PSLP种群.
- 用扫描和传输电子显微镜 (EM),流细胞计 (FC) 和纳米粒子追踪分析 (NTA) 来进行表征.
主要成果:
- PSLP 呈现多样化的形态和广泛的尺寸分布,随着储存时间的推移,小型和大型 PSLP 的度越来越高.
- 确定了三个不同的PSLP群体:脱粒血小板/幽灵/碎片,激活衍生PEV/有机体和PEV幽灵.
- 大多数PSLP表达了氨酸氨酸,诱导了血栓生成,含有细胞外线粒体,它们的释放动态因储存阶段而异.
结论:
- PSLP是一种异质的粒子组,包括脱粒的血小板,幽灵,碎片和细胞外囊泡.
- 与激活相关的PSLP主要在储存初期 (1-3天) 释放,而与亡/亡相关的PSLP则在晚些时候增加.
- 在较晚的存储阶段,细胞外线粒体的增加与血小板线粒消化无关.
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