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在减轻氨酸诱导的鱼中氧化损伤时,划分MAPK/NF-κB通路的参与,由氨酸通过氨酸

Mahammed Moniruzzaman¹, Saheli Kumar¹, Mainak Mukherjee²

¹Department of Zoology, University of Calcutta, Kolkata, India.

Environmental toxicology and pharmacology

|November 15, 2023

概括

黑色素通过恢复酶和抗氧化剂水平来保护鱼免受甲毒性. 它还调节关键的分子通路,包括NF-κB信号传递,减轻细胞损伤.

科学领域:

环境毒理学环境毒理学
生物化学生物化学
分子生物学分子生物学

背景情况:

甲是一种广泛使用的杀虫剂,可以对水生生物产生显著的毒性.
部组织是环境污染物的首要目标,导致生理功能受损.
黑激素是一种激素,以其抗氧化和保护性质而闻名.

研究的目的:

为了研究甲诱导的毒性分子机制在Notopterus notopterus.
为了评估黑激素对甲氨酸毒性的保护作用.
阐明转录因子和信号通路在甲氨酸毒性和黑激素改善作用中的作用.

主要方法:

成年Notopterus notopterus被暴露在不同度的甲基氨酸中,随后给予黑素.
在状组织上进行生物化学测试,以测量酶活性和氧化应激标志物.
分析了转录因子 (NF-κB,NRF2,Keap1) 和信号分子 (ERK,Akt,caspases) 的蛋白质表达.
在体外研究中,分离的状组织暴露于甲氨酸和黑激素.

主要成果:

甲暴露增加了损伤标志物 (MDA,XO,LDH) 和降低了必需酶活动 (Na+-K+-ATPase,SDH,细胞染色体C氧化酶).
抗氧化酶水平最初增加,然后随着度更高的甲基氨酸而下降.
黑素的使用逆转了这些生化变化,并恢复了正常的酶和抗氧化剂水平.
甲显著改变了NF-κB,NRF2,Keap1,ERK,Akt和caspases的表达,而这些表达被氨酸正常化.
黑色素的保护作用包括通过黑色素受体1调节酶蛋白和MAPK/NF-κB信号通路.

结论:

甲通过生物化学和分子破坏在鱼类中诱导显著的毒性.
黑色素有效地通过恢复生化平衡和调节关键分子通路来改善甲诱导的损伤.
这些发现突出了黑激素作为一种潜在的治疗剂,用于对抗水生生态系统中杀虫剂诱导的毒性.

关键词:

卡斯帕斯海域的卡斯帕斯海域细胞外信号调节的激酶.黑色素是什么黑色素是什么黑色素是什么这种动物叫 Notopterus notopterus.呼吸系统酶呼吸系统酶

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