长HKU4相关的冠状病毒在来自中国南部的大竹蝙蝠中发现
Min Guo1, Kai Zhao2, Xingwen Peng1
1Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, 510260, China.
Virologica Sinica
|November 15, 2023
概括
蝙蝠冠状病毒 (CoV) 在中国南部被发现,揭示了遗传多样性. 一个菌株,TyRo-CoV-162275,具有蛋白酶分裂部位,这是以前在蝙蝠HKU4r-CoV中未报告的特征.
科学领域:
- 病毒学 病毒学
- 动物传播疾病 动物传播疾病
- 基因组学就是基因组学.
背景情况:
- 蝙蝠是各种冠状病毒 (CoV) 的天然储存库.
- 像鱼这样的猎物动物的溢出事件是一个重大问题.
- 了解蝙蝠CoV多样性对于公共卫生至关重要.
研究的目的:
- 研究来自中国南部蝙蝠中的α冠状病毒 (αCoV) 和β冠状病毒 (βCoV) 的分子特征和遗传多样性.
- 识别新型冠状病毒并评估它们的动物传播潜力.
主要方法:
- 在云南和广东省的9个地点 (2016-2017年) 从20种蝙蝠物种收集了729个门拭子 (2016-2017年).
- 使用逆转录-聚合酶链反应 (RT-PCR) 检测CoV.
- 使用Illumina平台对代表性CoV菌株进行全基因组测序.
主要成果:
- 在六个地点的九种蝙蝠物种中检测到58个 (8.0%) 的蝙蝠CoV.
- 测序了两个全长基因组,即TyRo-CoV-162275和TyRo-CoV-162269,进行了测序.
- 泰罗-CoV-162275与松鼠相关的HKU4r-CoVs表现出相似性,并且在其S蛋白中具有蛋白酶裂解部位,这表明潜在的人类二二酶-4 (hDPP4) 受体使用.
- 泰罗-CoV-162269与先前确定的蝙蝠Cov.V.密切相关.
结论:
- 这项研究报告了第一个蝙蝠HKU4r-CoV菌株,具有furin蛋白酶分裂部位.
- 这些发现扩大了对Cov的地理分布和蝙蝠宿主范围的了解.
- 在TyRo-CoV-162275中存在素裂解部位,突出了潜在的动物传染性影响.
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