黄金葡萄球菌 (Staphylococcus aureus) 的pSK41/pGO1家族结合性等离子体对复制的密码抑制剂进行编码
Alvina Sarosh1, Stephen M Kwong1, Slade O Jensen2
1School of Life and Environmental Sciences, University of Sydney, Sydney, New South Wales 2006, Australia.
Plasmid
|November 15, 2023
概括
一种新发现的蛋白质Cop通过抑制复制基因的转录来调节金黄色葡萄球菌等离子体的复制. 这种蛋白质,与反意义RNAI一起,通过Cop控制等离子体拷贝数,尽管Cop控制了等离子体拷贝数.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 遗传学 遗传学 是一个
背景情况:
- 黄金葡萄球菌等离子体经常使用RepA_N蛋白质进行复制,其表达由反感RNAI控制.
- 像pSK41/pGO1这样的结合性等离子体在复制启动基因的上游具有额外的开放读取框架 (orf86).
- 预测Orf86是一个转录抑制因其螺旋-转-螺旋动态的转录抑制器.
研究的目的:
- 研究Orf86在调节pSK41等离子体促进体转录中的功能.
- 描述Orf86影响等离子体复制和拷贝数量的机制.
主要方法:
- 评估了Orf86对13个pSK41发起区的影响.
- 在试验室用纯化的His-Cop.进行了蛋白质-DNA结合试验.
- 确定了铜的晶体结构.
- 分析了Cop mRNA转录的起源.
主要成果:
- Orf86专门压制了来自代理发起人的转录,导致其重新命名为Cop.
- 过度表达Cop减少了pSK41迷你复制体的拷贝数,独立于RNAI.
- 警方将特定的DNA结合到靠近推广者的-35六合体的DNA上.
- 铜形成二元体,结构上与其他转录调节剂相似.
- Cop mRNA是由RNAI促进体的读透转录产生的.
结论:
- RNAI促进体转录了两种等离子体拷贝数的负调节体:反意义RNAI和Cop蛋白.
- Cop作为rep基因的转录抑制剂,补充RNAI的翻译抑制.
- 虽然Cop在复制数控制中发挥作用,但其在原生质粒中的删除表明其具有次要或辅助功能.
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