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脂肪酸脱和脂氧酶通路支持训练免疫力
Anaísa V Ferreira1,2, Juan Carlos Alarcon-Barrera3, Jorge Domínguez-Andrés4
1Department of Internal Medicine and Radboud Center for Infectious Diseases (RCI), Radboud University Nijmegen Medical Center, 6500HB, Nijmegen, The Netherlands. anaisa.validoferreira@radboudumc.nl.
Nature communications
|November 15, 2023
概括
巴塞勒斯卡尔梅特 - 格林 (BCG) 疫苗接种增强了先天性免疫细胞,建立了训练免疫力. 这涉及从单细胞中的多不和脂肪酸中增加的脂质媒介生物合成,这对于BCG诱导的反应至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 代谢途径 代谢途径
- 天生的免疫记忆天生的免疫记忆.
背景情况:
- 感染和疫苗可以诱导先天免疫细胞的长期变化,这种现象被称为训练免疫.
- 训练有素的免疫包括先天性免疫细胞的增强反应,为随后的挑战提供保护.
研究的目的:
- 调查脂质媒介体 (LM) 在人类单细胞中菌卡尔梅特-盖林 (BCG) 诱导的训练免疫力中的作用.
- 为了确定特定的脂质代谢途径,参与培训免疫的发展和功能.
主要方法:
- 来自健康个体的单细胞被暴露在BCG疫苗中.
- 使用药理学和遗传学方法来评估抑制脂质合成对训练免疫力的影响.
- 用生物化学测定测量脂质媒介和酶活性水平.
主要成果:
- 接种BCG疫苗的单细胞表现出由长链多不和脂肪酸 (PUFA) 衍生的脂质媒介的生物合成增加.
- 抑制长链PUFA合成和氧基酶衍生的LM损害了人体单细胞中BCG诱导的训练免疫力.
- 在BCG疫苗接种后的单细胞中,12-氧基酶活性产物升高.
结论:
- 脂质代谢途径,特别是涉及长链PUFA和脂氧酶活性的途径,对于BCG诱导的训练免疫是必不可少的.
- 了解这些脂质通路为训练免疫和调节先天免疫反应的潜在治疗点提供了洞察力.
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