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在纳米布沙漠土壤中,土壤抵抗体和移动体的表征
Yashini Naidoo1, Rian E Pierneef2, Don A Cowan3
1Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Lynnwood Road, Pretoria, 0002, South Africa. Yashini.naidoo@gmail.com.
概括
沙漠土壤含有多种抗生素耐药性基因 (ARG) 和移动遗传元素 (MGE). 横向基因转移,而不是细菌血统,推动了这些环境中耐药性的传播.
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 土壤抗体对理解抗生素耐药性的演变和在临床和环境环境之间传播至关重要.
- 对沙漠土壤抵抗体的知识仍然有限,这阻碍了对干旱生态系统中抵抗力学的全面理解.
研究的目的:
- 描述纳米布沙漠土壤中的细菌群落,抵抗体和移动体.
- 研究移动遗传元素和共同选择在抗生素耐药性基因 (ARG) 传播中的作用.
主要方法:
- 针对16S rRNA基因的定向测序,以分析细菌群体的组成.
- 枪支元基因组学用于识别抗生素耐药性基因 (ARG),金属/杀菌剂耐药性基因 (MRG/BRG) 和移动遗传元素 (MGE).
主要成果:
- 检测到各种ARG (埃尔法米辛,里法米辛,化),MRG/BRG (,铜) 和MGE (类似ColE1的等离子体).
- MRG/BRG和ARG的接近性表明了对抗性的潜在共同选择.
- 马氏体和水平获得的ARG的共存表明了细菌群体结构和ARG配置文件之间的脱.
结论:
- 纳米布沙漠的土壤细菌群体拥有各种各样的抗性元素.
- 横向基因转移在抗性元素的动态中起着重要作用,掩盖了宿主基因.
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