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识别和验证与自相关的基因在初级开角绿眼中

Wanjing Xu¹, Yuhao Sun², Shuang Zhao³

¹Ophthalmology Department of QingPu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China. xuwanjing23@163.com.

BMC medical genomics

|November 16, 2023

概括

两个关键的自相关基因,HSPA8和RPL15,在初级开角青光眼 (POAG) 中被确定. 这些基因可能通过调节自而影响POAG的进展,从而提供新的治疗点.

科学领域:

眼科医生眼科眼科
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

主要开角玻璃眼 (POAG) 是最常见的玻璃眼类型,但其确切原因尚不清楚.
自在POAG发育中起着作用,但具体的机制和目标需要进一步研究.

研究的目的:

在POAG.中识别差异表达的自相关基因 (DEARGs).
调查枢纽DEARGs在POAG发病过程中的作用及其与免疫细胞透和遗传变异的关系.

主要方法:

对GSE27276数据集和自基因组进行生物信息分析,以选DEARGs.
构建蛋白质-蛋白质相互作用网络并利用GSE138125数据集来识别枢纽DEARGs.
免疫细胞透分析,全基因组关联研究 (GWAS),基因组丰富分析 (GSEA) 和鼠POAG模型中的qRT-PCR验证.

主要成果:

确定了67个DEARG,其中HSPA8和RPL15被选为枢纽基因.
核心基因HSPA8和RPL15与免疫细胞透水平存在相关性.
对于HSPA8和RPL15,GWAS定位了与眼相关的区域,分别到11号和3号染色体.
GSEA揭示了与免疫,自,基因表达和HSPA8和RPL15.15的能量代谢相关的丰富途径.
qRT-PCR证实了Hspa8和Rpl15在老鼠POAG模型中的表达,与生物信息学发现保持一致.

结论:

HSPA8和RPL15涉及POAG的发病,可能通过自的调节.
这些发现为POAG机制提供了新的见解,并建议针对自的潜在治疗策略.

关键词:

自自是一种自的过程.生物信息学分析不同表达的基因.在HSPA8中,HSPA8是HSPA8标准.主要的开放角光眼.在RPL15中使用RPL15.这是RT-PCR.

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