曼诺斯合的金属有机框架通过PERK通路诱导瘤细胞热灭
Nianqiang Jin1, Binhang Wang2, Xinyao Liu2,3,4
1Department of Oral Pathology, School and Hospital of Stomatology, China Medical University, Shenyang, 110001, P. R. China.
Journal of nanobiotechnology
|November 16, 2023
概括
一种新型的曼诺糖修饰纳米层 (M-FNM) 有效地准瘤细胞,诱导热亡并增强抗瘤免疫力. 这种方法有望克服当前癌症免疫疗法的局限性.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 癌症研究 癌症研究
背景情况:
- 热症有可能增强抗瘤免疫反应.
- 现有的热致死诱导剂面临着药物耐药性,毒性和不良向等挑战.
- 需要新的治疗策略来克服癌症治疗中的这些局限性.
研究的目的:
- 开发一个多功能纳米球,精确地准瘤细胞.
- 为了在癌细胞中诱导有效的灭.
- 为了克服与当前热诱导剂相关的局限性.
主要方法:
- 改性金属有机框架 (MOF) 纳米球的合成:添加的Fe3O4@NH2-MIL-100 (M-FNM).
- 在CAL27细胞中通过曼诺酶受体 (MR) 介导的细胞内细胞结合体对M-FNM细胞吸收的研究.
- 对细胞内活性氧物种 (ROS) 生成,内等质网膜 (ER) 应激和PERK-eIF2α-ATF4-CHOP信号通路激活的分析.
- 在体内抗瘤疗效和免疫微环境调节的评估.
主要成果:
- 通过MR介导的内细胞分裂,M-FNM成功地进入了CAL27细胞,从而增加了细胞内ROS.
- 这种ROS激增触发了ER压力并激活了CHOP信号通路,导致Caspase-1激活和热灭症诱导.
- 在体内研究表明,M-FNM有效向瘤,具有显著的抗瘤作用,并促进T淋巴细胞透,重塑瘤免疫微环境.
结论:
- M-FNM显著抑制了瘤的生长.
- 这种基于M-FNM的新火诱导策略为开发有效的癌症免疫疗法提供了一个有希望的新方向.
更多相关视频
04:01Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
Published on: March 15, 2024
1.0K
06:12Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum
Published on: May 3, 2024
1.9K
相关概念视频
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
01:30Overview of Cell Death
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
01:31The Intrinsic Apoptotic Pathway
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...