通过生物工程的Corynebacterium glutamicum进行2'-fucosyllactose的新生合成
Ye-Gi Lee1,2, Hae-Yong Jo2, Do-Haeng Lee2
1Department of Bio and Fermentation Convergence Technology and Center for Bioconvergence, Kookmin University, Seoul, South Korea.
Biotechnology journal
|November 16, 2023
概括
生物技术生产的2'-Fucosyllactose (2'-FL),一个人乳寡糖,在工程 *Corynebacterium glutamicum*.实现. 这项研究优化了该过程,产生了21.5g L-1 2'-FL.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 代谢工程是代谢工程.
背景情况:
- 2缩写{'-Fucosyllactose (2缩写{'-FL) 是一种重要的母乳寡糖,具有显著的健康益处.
- 传统的合成方法很复杂;生物技术生产提供了一个可扩展的替代方案.
- *Corynebacterium glutamicum*是一种适合工业应用的GRAS (一般公认的安全) 生物.
研究的目的:
- 为了设计 *Corynebacterium glutamicum* 以实现 2 个缩写{'-FL. 的高效 de novo 生物合成.
- 优化发酵条件,以最大限度地提高产量和生产力.
- 建立一个强大的平台,用于生产其他母乳寡糖.
主要方法:
- 为GDP-L-fucose供应建立一个完整的新路径.
- 乳糖透酶和Helicobacter pylori*α-1,2-fucosyltransferase的异质表达方式.
- 遗传修饰包括2个缩写{'-FL出口者表达和pfkA基因破坏.
- 使用葡萄糖,果糖和乳糖优化料批量种植.
主要成果:
- 工程 *C. glutamicum* (BCGW_TL) 从葡萄糖中产生了0.25克L-1 2的缩写.
- 进一步的工程菌株 (BCGW_cTTLEΔP) 在批量培养中实现了2.5 g L-1 2 缩写{'-FL.
- 优化食批量种植的产量为21.5g L-1 2 缩写{'-FL},生产率为0.12g L-1 •h.
结论:
- *Corynebacterium glutamicum*是有效生物技术生产2缩写{'-FL.的可行的宿主.
- 开发的代谢工程和发酵策略显著提高了2缩写{'-FL产量.
- 这项工作为使用工程*C. glutamicum*生产其他有价值的人类牛奶寡糖的生产提供了基础.
关键词:
2′-FL出口商出口商的情况2′-fucosyllactose-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′-fucosyllactose 2′在Corynebacterium glutamicum的研究中,我们发现料批量种植种植方式α−1,2-fucosyl转移酶是什么更多相关视频
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