小麦质去化:结构,过敏性及其在低过敏面条中的应用
Mingxi Liu1, Shuhan Dai1, Lijun Yin1
1College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing, China.
Journal of the science of food and agriculture
|November 16, 2023
概括
酸去化通过改变蛋白质结构和减少IgE结合来降低小麦质 (WG) 的过敏性. 这种修改显示出开发低过敏性食品产品的前景,如面条.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 过敏性研究 研究过敏性研究
背景情况:
- 小麦质 (WG) 含有过敏原格莱丁和谷蛋白.
- 脱化将谷氨胺转化为谷氨酸,可能降低WG的过敏性.
研究的目的:
- 调查酸除化对小麦质 (WG) 结构,过敏性和面条质感的影响.
- 评估除化WG在低过敏性食品开发中的潜力.
主要方法:
- 在100°C下使用酸去amid化小麦质.
- 通过光谱 (FTIR,光) 和电泳 (SDS-PAGE) 方法分析结构变化.
- 与酶相关的免疫吸收试验 (ELISA) 和免疫血栓检测评估了过敏性.
- 脱化WG被用于准备复合面条.
主要成果:
- 脱amidation诱导了WG的结构展开和分子重量转移,主要影响低分子重量的谷蛋白子单元和γ-gliadin.
- 过敏性显著下降,由降低的IgE结合能力表明.
- 脱化WG (DWG-25) 改善了复合面条的纹理特性 (硬度,弹性,性,性).
结论:
- 酸去化有效降低小麦质的过敏性.
- 脱化WG具有开发新型低过敏性食品的潜力.
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