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使用擦拭刀片进行脂质双层改造,以改进离子通道分析

Hisatoshi Mimura1, Toshihisa Osaki1,2, Sho Takamori1

  • 1Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.

Analytical chemistry
|November 16, 2023
PubMed
概括

这项研究引入了一种新型装置,用于使用排列的脂质双层进行高通量离子通道分析. 一个独特的"擦拭刀"可以进行重复测量,大大提高了药物发现的数据吞吐量.

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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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