功能营养素对肠上皮细胞的作用,由氧化应激诱导
Hyun Woo Kim1, Seung Yun Lee2, Sun Jin Hur1
1Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Journal of animal science and technology
|November 16, 2023
概括
功能性营养素如氨酸,酸,甘氨酸和维生素C保护肠细胞免受氧化应激. 这些补充剂提高了细胞活力,并加强了的肠道屏障功能.
科学领域:
- 禽类的营养养方法
- 细胞生物学 细胞生物学
- 胃肠道的健康状况
背景情况:
- 氧化应激对肠上皮细胞 (cIECs) 有负面影响,损害了肠道健康和生产力.
- 功能营养素提供了潜在的保护机制,防止细胞损伤.
研究的目的:
- 评估特定氨基酸,维生素和矿物质对受氧化应激影响的CIECs的保护作用.
- 为了确定三,三,甘氨酸,维生素C,维生素E,维生素A,,和在减轻氧化损伤方面的有效性.
主要方法:
- 从没有特定病原体的蛋中分离CIEC.
- 用各种营养补充剂对细胞进行处理,然后暴露于过氧化 (H2O2),以诱导氧化应激.
- 评估细胞活力,使用FITC-dextran透性的肠道屏障功能,以及体电阻 (TEER).
主要成果:
- H2O2诱导的氧化应激显著降低了细胞活力和TEER,同时增加了肠道透性.
- 与维生素A,,和相比,氨酸,,甘氨酸,维生素C和维生素E在维持细胞活力方面表现出更好的保护.
- 三,甘氨酸,维生素C和的细胞透率最低,而氨酸,维生素E,维生素A,和也表现出保护作用.
- 氨酸,氨酸和甘氨酸显著改善了TEER值,其次是维生素C和维生素E,表明肠道屏障功能得到改善.
结论:
- 在氧化应激条件下的肠上皮细胞中,氨酸,三,甘氨酸和维生素C在改善细胞活力和肠壁功能方面是有效的.
- 这些功能营养素有望改善肠道健康和家禽生产的性能.
更多相关视频
04:53Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
30.6K
09:31Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
Published on: September 17, 2021
4.0K
相关概念视频
01:30Peroxisomes
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
01:16Necrosis
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
01:23Renewal of Intestinal Stem Cells
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
00:55Microvilli
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity to...
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity to...