精子干细胞保存和分化的3D培养系统开发的最新进展:叙述性综述
Leyla Fath-Bayati1, Leila Naserpour1,2, Mohadeseh Khoshandam2,3
1Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
International journal of reproductive biomedicine
|November 16, 2023
概括
保护男性生育能力至关重要,特别是当不孕症没有明确的原因时. 三维细胞培养显示出保留精子和精子干细胞的前景,有助于它们的分化和男性生殖功能的潜在恢复.
科学领域:
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
背景情况:
- 男性不孕症影响了相当一部分人口,通常是由于未知的原因.
- 保存精子和精子干细胞 (SSCs) 的有效方法对于维持男性生育能力至关重要.
- 目前的保存技术需要优化,以便在体内和体外环境中长期有效.
研究的目的:
- 审查目前精子和SSC保存和体外培养的方法.
- 评估不同技术对细胞分化和存活的影响.
- 确定有希望的生物工程系统来维持男性生育能力.
主要方法:
- 从1973年到2022年进行了一项全面的MEDLINE/PubMed搜索.
- 关键词包括"精子原始细胞"",干细胞"",生育保存"",冷精子"",细胞分化"",组织支架"和"三维细胞培养".
- 添加了相关文章,并排除了非英语,仅摘要和无关文章.
主要成果:
- 现代生物工程系统为男性生育能力的保护提供了新的希望.
- 很少有体外精子生成研究成功地产生了大量成熟的雌性体.
- 三维细胞培养表明对SSC分化和保存的影响最大.
结论:
- 精子的保存和SSC的分化是关键的研究领域.
- 存在各种方法,每个方法都有优点和缺点.
- 模仿细胞外基质的组织支架支持胚胎细胞的发育和生存,3D培养尤其具有影响力.
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