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Updated: Jul 11, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

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在大肠杆菌中,生物合成和对非水解素转化为重组蛋白的遗传编码

Phillip Zhu1,2, Ryan A Mehl1,2, Richard B Cooley1,2

  • 1Department of Biochemistry and Biophysics, Oregon State University, 2011 Agricultural and Life Sciences, Corvallis, OR, USA.

Bio-protocol
|November 16, 2023
PubMed
概括

研究人员开发了一种高效的大肠杆菌表达系统,用于局部特定的非水解素 (nhpSer) 模仿物. 这种PermaPhos系统克服了蛋白质脱的挑战,提高了研究酸化依赖相互作用的产量超过40倍.

关键词:
珀的抑制抑制珀的抑制生物合成途径 生物合成途径遗传密码扩展 遗传密码扩展非可水解的素.永恒 (PermaPhos) 是一种常见的.索氨酸是一种素.再组合蛋白质的表达方式

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