血单聚氨酸耗尽对血型独立的血输血
Johannes Raster1, Michelle Jacob1, Andreas Greinacher1
1Institut für Immunologie und Transfusionsmedizin, Abteilung Transfusionsmedizin, Universitätsmedizin Greifswald, Greifswald, Germany.
概括
我们开发了一种方法,使用红细胞从血中去除抗A和抗B抗体. 这项创新使得血型独立的血输血成为可能,这对于紧急情况和克服供应短缺至关重要.
科学领域:
- 输血医学 输血医学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 血输血对于严重的血液损失至关重要,但ABO兼容性是一个限制.
- 血中的抗A和抗B抗体需要血液组匹配.
- 这一要求限制了在紧急情况下使用等离子体,并可能导致短缺.
研究的目的:
- 开发一种方法来从血中消耗抗A和抗B异凝素.
- 为了使 ABO 血型独立的血输血.
主要方法:
- 新鲜冷血被聚合并与红细胞一起化,以吸附异凝素.
- 通过离心去除抗体-红细胞复合体.
- 耗尽的血被测试为异凝聚素标位,自由血红蛋白,残留红细胞,凝结因子活性和不孕.
主要成果:
- 在A组血中的抗B titre降至1:1或更低;在B组血中的抗A titre降至至少1:16.
- 凝血因子活动 (V,VIII,XI) 保持在84%以上.
- 没有检测到剩余的红细胞,自由血红蛋白的增加很小;所有单位仍然是无菌的.
结论:
- 成功开发了一种生产抗A和抗B枯竭血的封闭系统方法.
- 这种缺少异凝素的血可能有助于进行血型独立的输血.
- 该方法使用标准设备,并解决了紧急血可用性的局限性.
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