转录因子HBF1直接激活多个开花时间抑制剂的表达,以延迟大米开花
Cong Li1,2, Liya Zhang1, Xin Wang1
1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
aBIOTECH
|November 16, 2023
概括
米的开花时间由Hd3a结合抑制因子1 (HBF1) 调节. 通过向关键的开花抑制基因,增强HBF1活动延迟了开花,揭示了新的分子机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物遗传学 植物遗传学
- 农业学是一种农业学.
背景情况:
- 开花时间是一个关键的农学特征,影响作物的适应性和产量.
- Hd3a结合抑制因子1 (HBF1) 是一种已知的延迟开花的转录因子.
- 在花期调节中的HBF1精确分子机制仍然不完全理解.
研究的目的:
- 阐明HBF1调节大米开花时间的分子机制.
- 使用混合转录因子 (HTF) 策略来增强HBF1的转录活性.
- 为了识别HBF1的下游点基因,这些基因参与了开花控制.
主要方法:
- 利用混合转录因子 (HTF) 策略创建HBF1-VP64 (HBF1V) 转基因大米系.
- 在不同的光周期下分析了HBF1V,HBF1-MYC和野生类型大米的开花时间.
- 进行了转录组深度测序,以确定HBF1V系中差异表达的基因.
- 进行了实验,以验证HBF1.1的直接标基因.
主要成果:
- 与对照组相比,过度表达HBF1-VP64 (HBF1V) 的转基因大米表现出明显延迟的开花.
- 由HBF1V引起的延迟开花通过Ehd1-Hd3a/RFT1通路运行.
- 转录组分析显示,在HBF1V系中,有19个WRKY家族基因的上调.
- 确定了OsWRKY64,DTH7,OsCO3和OsWRKY104作为HBF1的直接下游目标,抑制了开花.
结论:
- 增强的HBF1活动有效地延迟了大米的开花时间.
- HBF1直接准并调节多个开花抑制基因,包括OsWRKY64,DTH7,OsCO3和OsWRKY104.
- 这项研究提供了一个详细的分子框架,用于HBF1介导的调节水的开花时间.
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