Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Meiosis II02:02

Meiosis II

45.8K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.8K
Cohesins02:20

Cohesins

4.5K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.5K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Condensins02:15

Condensins

3.5K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dysfunction of a SET3-like complex underlies a family of related neurological disorders.

Nature communications·2026
Same author

Hydrogeochemical Characteristics and Quality Assessment of Groundwater in Xingtai Selenium-Enriched Area, Central North China.

Water environment research : a research publication of the Water Environment Federation·2026
Same author

Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy.

eLife·2026
Same author

Defining the chromatin-associated protein landscapes on <i>Trypanosoma brucei</i> repetitive elements using synthetic TALE proteins.

eLife·2026
Same author

A nowhere-to-hide mechanism ensures complete piRNA-directed DNA methylation.

Nature·2026
Same author

Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity.

The EMBO journal·2025

相关实验视频

Updated: Jul 11, 2025

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

9.4K

SUMOylation 调节了Lem2 功能的中心分子聚类和沉默.

Joanna Strachan1, Orsolya Leidecker2, Christos Spanos3

  • 1Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, UK.

Journal of cell science
|November 16, 2023
PubMed
概括

对SUMOylation酶的空间控制至关重要. 在裂变酵母中,局部错误的SUMO蛋白酶Ulp1由于外周超SUMOylation导致中心膜缺陷,影响沉默并促进聚类.

关键词:
这种植物是Schizosaccharomyces pombe.中介质是中介质的组成部分.这就是Lem2 Lem2 Lem2这就是SUMOylation的作用.无处不在的关系

更多相关视频

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

12.2K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K

相关实验视频

Last Updated: Jul 11, 2025

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

9.4K
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

12.2K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 通过小型基因类修饰剂 (SUMO) 结合的调节对细胞过程至关重要.
  • SUMOylation酶 (SUMOylation和deSUMOylation) 的空间定位对于基质修饰至关重要.
  • 内核膜蛋白Lem2参与了中间体的功能,包括沉默和聚类.

研究的目的:

  • 为了研究空间SUMOylation在裂变酵母中中粒体调节中的作用.
  • 阐明 SUMO 蛋白酶 Ulp1 局部化影响中心函数的机制.
  • 了解Lem2在中间体聚类和沉默中的不同角色是如何协调的.

主要方法:

  • 使用了裂变酵母Schizosaccharomyces pombe作为一个模型生物体.
  • 研究了从核外移位SUMO蛋白酶Ulp1的影响.
  • 分析了中间体缺陷,核外围的超SUMOylation,中间体沉默和中间体聚类.
  • 研究了内核膜蛋白Lem2的SUMOylation的作用.

主要成果:

  • Ulp1的外定位导致核外围的超SUMOylation,导致中心体缺陷.
  • 局部化的超-SUMOylation 损害了中间体沉默,但增强了中间体聚类.
  • 这两种效应都部分依赖于Lem2.2的SUMOylation.
  • 莱姆2的SUMOylation似乎作为一个调节开关,平衡其在替代途径中的角色.

结论:

  • SUMOylation 在空间调节中位素功能的过程中起着至关重要的作用.
  • 莱姆2在中间体聚类和沉默中的独特功能是通过SUMOylation调节的.
  • SUMOylation 是一个关键的调节器,协调Lem2的相互作用,以平衡其在不同细胞途径中的作用.
  • 这项研究揭示了SUMOylation在促进中间体聚类中的新角色.